Fluoromount-G熒光封片劑（不含不含DAPI）

產(chǎn)品描述：Fluoromount-G?封片劑是一種水溶性化合物，該熒光封片劑水溶性好，可在實驗最后封片時使用。由于Fluoromount-G?封片劑是水溶性的，因此可將載玻片浸入PBS溶液中，直到蓋玻片松開，從而移開蓋玻片。使用該熒光封片劑可以減少熒光淬滅，便于更好的分析實驗結(jié)果。
產(chǎn)品組分：PBS,磷酸鹽緩沖液，甘油，0.21%疊氮鈉
體積：25ml
保存：22-25℃
應用：免疫組化-冰凍切片
      免疫組化-石蠟切片
      原位雜交
      細胞凋亡
產(chǎn)品訂購：

封片是將組織切片封固保存于載玻片與蓋玻片之間,使之不與空氣發(fā)生接觸，防止其氧化、褪色,利于鏡檢觀察及保存。不可逆的光漂白會造成熒光信號的損失,從而導致靈敏度降低,尤其在靶標分子豐度較低、激發(fā)光較強或激發(fā)持續(xù)時間長時，表現(xiàn)得非常明顯。SouthernBiotech公司專i ]開發(fā)出的Fluorescent-G系列是抗熒光淬滅封片劑為您解決熒光信號淬滅問題。該系列熒光封片劑水溶性好,包括偶聯(lián)DAPI熒光封片劑和不帶偶聯(lián)的熒光封片劑,可在實驗最后后封片時使用。使用該偶聯(lián)DAPI熒光封片劑不僅可以減少熒光淬滅,還可同時將細胞核染成藍色,以將封片和復染-步完成。便于更好的分析實驗結(jié)果。

點擊查看產(chǎn)品說明書：

操作流程：

1、準備好染色后的切片或爬片。

2、去除樣本周圍多余的液體,加入1滴(~50ul)封片劑,覆蓋區(qū)域22x22mm。

3、蓋上蓋玻片,去除多余的封片劑。

4、空氣中晾干5min后檢測。

產(chǎn)品應用：

適用于組織切片、細胞圖片/爬片

實例

參考文獻：

1、Casas C, Isus L, Herrando-Grabulosa M, Mancuso FM, Borrás E, Sabidó E, et al. Network-based proteomic approaches reveal the neurodegenerative, neuroprotective and pain-related mechanisms involved after retrograde axonal damage. Sci Rep. 2015;5:9185. (IHC-FS, CY2, CY3, DAPI, NeuroTrace? 435/455 Blue)

2、Cheng C, Wang W, Huang C, Du P, Yang J, Tsaur M. Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons. J Comp Neurol. 2016;524:846-73. (IHC-FS, AF488, AF594, AF647, TSA? CY3)

3、Cursio R, Miele C, Filippa N, Colosetti P, Auberger P, Van Obberghen E, et al. Tyrosine phosphorylation of insulin receptor substrates during ischemia/reperfusion-induced apoptosis in rat liver. Langenbecks Arch Surg. 2009;394:123-31. (IHC-FS, DAPI, Evans Blue, FLIVO?)

4、del Mar N, von Buttlar X, Yu AS, Guley NH, Reiner A, Honig MG. A novel closed-body model of spinal cord injury caused by high-pressure air blasts produces extensive axonal injury and motor impairments. Exp Neurol. 2015;271:53-71. (IHC-FS, AF488, AF594, AF647, EYFP)

5、Elsner RA, Hastey CJ, Olsen KJ, Baumgarth N. Suppression of long-lived humoral immunity following Borrelia burgdorferi infection. PLoS Pathog. 2015;11(17):e1004976. (IHC-FS, AF594, eFluor? 450, FITC)

6、Gaillard D, Xu M, Liu F, Millar SE, Barlow LA. β-catenin signaling biases multipotent lingual epithelial progenitors to differentiate and acquire specific taste cell fates. PLoS Genet. 2015;11(5):e1005208. (IHC-FS, ISH, TUNEL, AF488, AF546, BCIP/NBT, DRAQ5?, SYTOX? Green, TMR Red, X-gal, YFP)

7、Herbig ME, Houdek P, Gorissen S, Zorn-Kruppa M, Wladykowski E, Volksdorf T, et al. A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin. Eur J Pharm Biopharm. 2015;95:99-109. (IHC-FS, IHC-PS, AF488, DAPI, MTT, Texas Red?)

8、Hilton BJ, Assinck P, Duncan GJ, Lu D, Lo S, Tetzlaff W. Dorsolateral funiculus lesioning of the mouse cervical spinal cord at C4 but not at C6 results in sustained forelimb motor deficits. J Neurotrauma. 2013;30:1070-83. (IHC-FS, CY3, DyLight? 405)

9、Imai DM, Feng S, Hodzic E, Barthold SW. Dynamics of connective-tissue localization during chronic Borrelia burgdorferi infection. Lab Invest. 2013;93:900-10. (IHC-FS, DAPI, NorthernLights? 493)

10、Kim YU, Lim H, Jung HE, Wetsel RA, Chung Y. Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS One. 2015;10(3):e0120294. (IHC-FS, DyLight? 594, DyLight? 650, Image-iT? FX Signal Enhancer , Pacific Blue?)

11、Lang J, Ota T, Kelly M, Strauch P, Freed BM, Torres RM, et al. Receptor editing and genetic variability in human autoreactive B cells. J Exp Med. 2016;213:93-108. (IHC-FS, APC, DAPI, eFluor? 615)

12、Laver CR, Metcalfe AL, Szczygiel L, Yanai A, Sarunic MV, Gregory-Evans K. Bimodal in vivo imaging provides early assessment of stem-cell-based photoreceptor engraftment. Eye. 2015;29:681-90. (IHC-FS, ICC, NorthernLights? 557, Qtracker? 655)

13、Lee CS, Hanna AD, Wang H, Dagnino-Acosta A, Joshi AD, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659. (IHC-FS, AF488, AF594, DAPI, Heochst 33342, PROTEOSTAT?)

14、Lee J, Kapur M, Li M, Choi M, Choi S, Kim H, et al. MFN1 deacetylation activates adaptive mitochondrial fusion and protects metabolically challenged mitochondria. J Cell Sci. 2014;127:4954-63. (IHC-FS, DAB, NBT)

15、Marsh SE, Abud EM, Lakatos A, Karimzadeh A, Yeung ST, Davtyan H, et al. The adaptive immune system restrains Alzheimer’s disease pathogenesis by modulating microglial function. Proc Natl Acad Sci USA. 2016;113:E1316-25. (IHC-FS, AF488, AF555, Amylo-Glo?)

16、McBride EG, Rubel EW, Wang Y. Afferent regulation of chicken auditory brainstem neurons: rapid changes in phosphorylation of elongation factor 2. J Comp Neurol. 2013;521:1165-83. (IHC-FS, AF488, AF568, NeuroTrace? 640/660 Deep-Red)

17、McClure S, Dudler L, Thorpe D, Hein WR. Analysis of cell division among subpopulations of lymphoid cells in sheep. I. Thymocytes. Immunology. 1988;65:393-9. (IHC-FS, ICC, DAB, Fast Red, Fast Blue, Hematoxylin)

18、Tran HP, Shin E, Saito K, Nguyen XT, Chung YH, Jeong JH, et al. Protective potential of IL-6 against trimethyltin-induced neurotoxicity in vivo. Free Radic Biol Med. 2012;52:1159-74. (IHC-FS, AF488, AF594, Fluoro-Jade?)

19、Michard E, Lima PT, Borges F, Silva AC, Portes MT, Carvalho JE, et al. Glutamate receptor-like genes form Ca2+ channels in pollen tubes and are regulated by pistil D-serine. Science. 2011;332:434-7. (IHC-FS, DRAQ5?, Marina Blue?)

20、Murphy S, Henry M, Meleady P, Zweyer M, Mundegar RR, Swandulla D, et al. Simultaneous pathoproteomic evaluation of the dystrophin-glycoprotein complex and secondary changes in the mdx-4cv mouse model of Duchenne muscular dystrophy. Biology. 2015;4:397-423. (IHC-FS, AF488, Hoechst 33342, Rhodamine Red?-X, Sudan Black B)

21、Piekarski N, Gross JB, Hanken J. Evolutionary innovation and conservation in the embryonic derivation of the vertebrate skull. Nat Commun. 2014;5:5661. (IHC-FS, AF488, Alizarin Red S, DAPI)

22、Qvartskhava N, Lang PA, G?rg B, Pozdeev VI, Ortiz MP, Lang KS, et al. Hyperammonemia in gene-targeted mice lacking functional hepatic glutamine synthetase. Proc Natl Acad Sci USA. 2015;112:5521-6. (IHC-FS, CY3, Eosin, FITC, Hematoxylin, Hoechst 34580)

23、Sic H, Kraus H, Madl J, Flittner K, von Münchow AL, Pieper K, et al. Sphingosine-1-phosphate receptors control B-cell migration through signaling components associated with primary immunodeficiencies, chronic lymphocytic leukemia, and multiple sclerosis. J Allergy Clin Immunol. 2014;134:420-8. (IHC-FS, AF405, AF568, AF594, AmCyan, FITC)

24、Stone LM, Tan S, Tam PP, Finger TE. Analysis of cell lineage relationships in taste buds. J Neurosci. 2002;22:4522-9. (IHC-FS, CY5, DAPI, FITC, Lissamine? Rhodamine B, PI)

25、Szabadfi K, Estrada C, Fernandez-Villalba E, Tarragon E, Setalo G Jr, Izura V, et al. Retinal aging in the diurnal Chilean rodent (Octodon degus): histological, ultrastructural and neurochemical alterations of the vertical information processing pathway. Front Cell Neurosci. 2015;9:126. (IHC-FS, AF488, AF568, CY3.5, DAPI, FITC)

26、Tan X, Xue Y, Ma T, Wang X, Li JJ, Lan L, et al. Partial eNOS deficiency causes spontaneous thrombotic cerebral infarction, amyloid angiopathy and cognitive impairment. Mol Neurodegener. 2015;10:24. (IHC-FS, AF488, AF555, AF594, DAPI, DyLight? 488, Fluoro-Emerald, Thioflavin S)

27、Wang H, Liu N, Zhang YP, Deng L, Lu Q, Shields CB, et al. Treadmill training induced lumbar motoneuron dendritic plasticity and behavior recovery in adult rats after a thoracic contusive spinal cord injury. Exp Neurol. 2015;271:368-78. (IHC-FS, Fast Blue, FITC, Fluoro-Gold, TRITC)

28、Watanabe K, Nishimura Y, Oka T, Nomoto T, Kon T, Shintou T, et al. In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives. BMC Neurosci. 2010;11:116. (IHC-FS, AF488, Coumarin)

29、Choi VW, Bigelow CE, McGee TL, Gujar AN, Li H, Hanks SM, et al. AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice. Mol Ther Methods Clin Dev. 2015;2:15022. (IHC-PS, AEC, Hematoxylin)

30、Han JJ, Mhatre AN, Wareing M, Pettis R, Gao W, Zufferey RN, et al. Transgene expression in the guinea pig cochlea mediated by a lentivirus-derived gene transfer vector. Hum Gene Ther. 1999;10:1867-73. (IHC-PS, ICC, AMCA, GFP, Texas Red?)